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[[File: Pope Clement VII.jpg|thumbnail|200px|left|Pope Clement VII in 1528]]
Emperor Charles V was deeply embarrassed by the actions of his mutinous army. However, Charles knew that the Pope was in a weak position and he saw it as an opportunity to extend his control over the Papacy.<ref> Chastel. p. 212</ref>
. Successive Popes, eager to preserve Italian independence and their own had allied themselves with the French, to prevent Charles from upsetting the balance of power in Italy . Charles V now used the weakened position of Pope Clement to ensure that the Papacy was no longer able to resist Imperial interests in Italy. After the Sack of Rome, Pope Clement was too afraid of Charles V after the Sack to adopt a policy that was independent of the Emperor. This policy was to have momentous consequences not only for the Church but also for the history of Europe. In the aftermath of the Sack, the Popes were very reluctant to go against the wishes of the Emperor and after his abdication, the Spanish monarchs, who inherited the greatest part of Charles V territories<ref> Tuchman, p. 347</ref>.
The Popes insistence on religious orthodoxy meant that freethought and secular values were increasingly challenged in the aftermath of the Sack of Rome. The Inquisition became more active, as the Pope sought to stamp out every sign of free thought or ideas that were contrary to the teachings of the Church. The growing fear of Protestantism was also instrumental in the new climate but the fear of the Emperor after the Sack and the Spanish meant that the Inquisition became all-pervasive in Italian society. This was to have a devastating impact on the Renaissance. This cultural flourishing was premised on an attempt to reproduce the classical world. It was
largely humanistic, secular and often overtly pagan and this was no longer possible in the aftermath of the capture of Rome in 1527. It became increasingly difficult for the humanist who were once so influential to express their views and instead of studying the ancient classics they were expected to study religious works.<ref> Burckhardt, p. 120</ref> No longer could they think and write freely as by the mid-1500s they were intimidated by the inquisition. The new emphasis on religious orthodoxy meant that many noble patrons were unwilling to subsidize the works of humanists who expressed ‘pagan’ ideas.